Structure of DNA & RNA FYBSc Microbiology MCQs Set-1

MCQues DNA RNA Questions Answers

1:A peculiar cytochrome is observed in bacteria and it can react with molecular oxygen, what is it? 

(a) Cyt b 
(b) Cyt c 
(c) Cyt d 
(d) Cyt o

Answer:(d) Cyt o

2:The genetic material in HIV is 

(a) ds DNA 
(b) ss DNA 
(c) s RNA 
(d) None of these

Answer:(a) ds DNA 

3:Which one of the following mutagens act only on replicating DNA? 

(a) Ethidium bromide 
(b) Nitrosoguanidine 
(c) Acridine orange 
(d) None of above

Answer:(c) Acridine orange 

4:Poly A tail is frequently found in 

(a) Histone in RNA 
(b) Bacterial RNA 
(c) eukaryotic RNA 
(d) TRNA

Answer: (c) eukaryotic RNA 

5:Which of the following is an example of RNA virus? 

(a) SV 40 
(b) T4 phage 
(c) Tobacco mosaic virus 
(d) Adenovirus

Answer:(c) Tobacco mosaic virus 

6:Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called 

(a) Clones 
(b) Genomic library 
(c) mRNA 
(d) None of these

Answer:(b) Genomic library 

7:Transgenic animals are produced when GH gene fused with 

(a) MT gene 
(b) GH 
(c) GRF 
(d) FIX

Answer:(a) MT gene 

8:In which medium the hybridoma cells grow selectively? 

(a) Polyethylene glycol 
(b) Hypoxanthine aminopterin thymidine 
(c) Hypoxanthine-guanine phosphoribosyl transferase 
(d) Both b and c

Answer:(b) Hypoxanthine aminopterin thymidine 

9:The enzymes which are commonly used in genetic engineering are 

(a) Exonuclease and ligase 
(b) Restriction endonuclease and polymerase 
(c) Ligase and polymerase 
(d) Restriction endonuclease and ligase

Answer:(a) Exonuclease and ligase 

10:A successful hybridoma was produced by fusing 

(a) Plasma cells and plasmids 
(b) Plasma cells and myeloma cells 
(c) Myeloma cells and plasmids 
(d) Plasma cells and bacterial cells

Answer:(b) Plasma cells and myeloma cells 

11:The technique involved in comparing the DNA components of two samples is known as 

(a) Monoclonal antibody techniques 
(b) Genetic finger-printing 
(c) Recombinant DNA technology 
(d) Polymerase chain reaction

Answer:(b) Genetic finger-printing 

12:Plasmids are ideal vectors for gene cloning as 

(a) They can be multiplied by culturing
(b) They can be multiplied in the laboratory using enzymes 
(c) They can replicate freely outside the bacterial cell 
(d) They are self replicating within the bacterial cell

Answer:(d) They are self replicating within the bacterial cell

13:Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell? 

(a) 46 
(b) 23 
(c) 47 
(d) 44 

Answer:(d) 44 

14:Pasteur effect is due to 

(a) Change from aerobic to anaerobic 
(b) Providing oxygen to anaerobically respiring structures 
(c) Rapid utilization of ATP 
(d) Non synthesis of ATP

Answer:(b) Providing oxygen to anaerobically respiring structures 

15:A mechanism that can cause a gene to move from one linkage group to another is 

(a) Trans location 
(b) Inversion 
(c) Crossing over 
(d) Duplication

Answer:(a) Trans location 

16:The smallest unit of genetic material that can undergo mutation is called 

(a) Gene 
(b) Cistron 
(c) Replicon 
(d) Muton

Answer:(d) Muton

17:The two chromatids of metaphase chromosome represent 

(a) Replicated chromosomes to be separated at anaphase 
(b) Homologous chromosomes of a diploid set 
(c) Non-homologous chromosomes joined at the centromere 
(d) Maternal and paternal chromosomes joined at the centromere

Answer:(a) Replicated chromosomes to be separated at anaphase 

18:Malate dehydrogenase enzyme is a 

(a) Transferase 
(b) Hydrolase 
(c) Isomerase 
(d) Oxidoreductase

Answer:(d) Oxidoreductase

19:In E.Coli att site is in between 

(a) Gal and biogenes
(b) Bio and niacin genes 
(c) Gal and B genes 
(d) None of these

Answer:(a) Gal and biogenes

20:The best vector for gene cloning 

(a) Relaxed control plasmid 
(b) Stringent control plasmid
(c) Both a and b 
(d) None of these

Answer:(a) Relaxed control plasmid 

21:A gene that takes part in the synthesis of polypeptide is 

(a) Structural gene 
(b) Regulator gene 
(c) Operator gene 
(d) Promoter gene 

Answer:(a) Structural gene 

22:DNA replicates during 

(a) G1 – phase 
(b) S – phase 
(c) G2 – phase 
(d) M – phase

Answer:(b) S – phase 

23:A human cell containing 22 autosome and a ‘Y’ chromosome is probably a 

(a) Male somatic cell 
(b) Zygote 
(c) Female somatic cell 
(d) Sperm cell

Answer:(b) Zygote 

24:Crossing-over most commonly occurs during 

(a) Prophase I 
(b) Prophase II 
(c) Anaphase I 
(d) Telophase II

Answer:(a) Prophase I 

25:DNA-replication is by the mechanism of 

(a) Conservative 
(b) Semiconservative 
(c) Dispersive 
(d) None of the above

Answer:(b) Semiconservative 

26:Production of RNA from DNA is called

(a) Translation 
(b) RNA splicing 
(c) Transcription 
(d) Transposition

Answer:(c) Transcription 

27:Nucleic acids contain 

(a) Alanine 
(b) Adenine 
(c) Lysine 
(d) Arginine

Answer:(b) Adenine 

28:What are the structural units of nucleic acids? 

(a) N-bases 
(b) Nucleosides 
(c) Nucleotides 
(d) Histones

Answer:(c) Nucleotides 

29:The most important function of a gene is to synthesize 

(a) Enzymes 
(b) Hormones 
(c) RNA 
(d) DNA

Answer:(a) Enzymes 

30:One of the genes present exclusively on the X-chromosome in humans is concerned with 

(a) Baldness 
(b) Red-green colour baldness 
(c) Facial hair/moustache in males 
(d) Night blindness

Answer:(b) Red-green colour baldness